Magdy S. Alabady, PhD, MSc

Faculty, Scientist, and Director



Department of Plant Biology

University of Georgia Athens

Address 1:
Department of Plant Biology
2502 Miller Plant Sciences
University of Georgia
Athens, GA 30602

Address 2:
Georgia Genomics and Bioinformatics Lab
110 Riverbend Rd., Room 161
Athens, GA 30602



Serum albumin alters the expression of iron-controlled genes in Pseudomonas aeruginosa.


Journal article


C. Kruczek, M. Wachtel, Magdy S. Alabady, P. Payton, J. Colmer-Hamood, A. Hamood
Microbiology, 2012

Semantic Scholar DOI PubMed
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APA   Click to copy
Kruczek, C., Wachtel, M., Alabady, M. S., Payton, P., Colmer-Hamood, J., & Hamood, A. (2012). Serum albumin alters the expression of iron-controlled genes in Pseudomonas aeruginosa. Microbiology.


Chicago/Turabian   Click to copy
Kruczek, C., M. Wachtel, Magdy S. Alabady, P. Payton, J. Colmer-Hamood, and A. Hamood. “Serum Albumin Alters the Expression of Iron-Controlled Genes in Pseudomonas Aeruginosa.” Microbiology (2012).


MLA   Click to copy
Kruczek, C., et al. “Serum Albumin Alters the Expression of Iron-Controlled Genes in Pseudomonas Aeruginosa.” Microbiology, 2012.


BibTeX   Click to copy

@article{c2012a,
  title = {Serum albumin alters the expression of iron-controlled genes in Pseudomonas aeruginosa.},
  year = {2012},
  journal = {Microbiology},
  author = {Kruczek, C. and Wachtel, M. and Alabady, Magdy S. and Payton, P. and Colmer-Hamood, J. and Hamood, A.}
}

Abstract

Pseudomonas aeruginosa, which causes serious infections in immunocompromised patients, produces numerous virulence factors, including exotoxin A and the siderophore pyoverdine. As production of these virulence factors is influenced by the host environment, we examined the effect serum has on global transcription within P. aeruginosa strain PAO1 at different phases of growth in an iron-deficient medium. At early exponential phase, serum significantly enhanced expression of 138 genes, most of which are repressed by iron, including pvdS, regA and the pyoverdine synthesis genes. However, serum did not interfere with the repression of these genes by iron. Serum enhanced regA expression in a fur mutant of PAO1 but not in a pvdS mutant. The serum iron-binding protein apotransferrin, but not ferritin, enhanced regA and pvdS expression. However, in PAO1 grown in a chemically defined medium that contains no iron, serum but not apotransferrin enhanced pvdS and regA expression. While complement inactivation failed to eliminate this effect, albumin absorption reduced the effect of serum on pvdS and regA expression in the iron-deficient medium chelexed tryptic soy broth dialysate. Additionally, albumin absorption eliminated the effect of serum on pvdS and regA expression in the chemically defined medium. These results suggest that serum enhances the expression of P. aeruginosa iron-controlled genes by two mechanisms: one through apotransferrin and another one through albumin.





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